VIB Nanobody Core

Nanobodies® are an extremely useful class of molecules for research, diagnostics and therapeutics in human and animal health. You can use them in a wide variety of fields, such as immunohistochemistry, immunomodulation, intracellular expression (intrabodies) and biosensor-applications. As proteomics tools, you can deploy them for expression profiling, physical mapping of proteins, protein-protein interaction studies, functional analysis and (in)activation of genens. Developed at the Vrije Universiteit Brussel, Belgium, the powerful Nanobody® technology is now available to both academic as to non-academic researchers through the VIB Nanobody Core.

Deadline for the receipt of antigen for the upcoming immunization:

8 November 2017

Services

Nanobodies®

To obtain Nanobodies®, the researcher provides the VB Nanobody Core with a sufficiently pure protein or peptide (~0.5 mg).

  • The facility delivers 1 to 10 different Nanobodies® for a given antigen.
  • Nanobodies® that score positive in ELISA will be supplied as bacterial clones.
  • The researcher also obtains the antigen binding sequence of each Nanobody®.
  • The affinity of the Nanobody® will be in the range of 0.1 to 100 nM for proteinaceous antigens or from 0.1 to 10 µM for peptidic/haptenic antigens.
  • Timeline: about 3 months from immunization until delivery of Nanobodies®.
  • The success-rate for the entire procedure is about 70-80%. The scientist will only be charged after the successful delivery of a Nanobody®.

Additional services

In addition to the delivery of standard Nanobodies®, additional services can be offered:

  • Determination and ranking of affinity (kon, koff, KD).
  • Engineering towards higher stability, affinity.
  • Expression vectors are available to express modified recombinant constructs:
    • Nanobodies® can be cloned in front of a human or mouse IgG domain (or any other animal/isotype).
    • Nanobodies® can be tagged with Flag, c-myc, His, Cy-dyes, FITC, GFP, lactamase etc.
    • Nanobodies® can be biotinylated for use in immunoprecipitation applications with streptavidin beads.
    • Nanobodies® can be engineered into bivalent or bispecific antibody constructs.

Team 

NSF consists of the following members:

Contact 

Address

VIB Nanobody Core, Vrije Universiteit Brussel
Department of Molecular and Cellular Interactions
Vrije Universiteit Brussel
Pleinlaan 2
1050 Brussel - Belgium

Tel: +32 (0)2 629 19 78

Fax: +32 (0)2 629 19 81